CryoEM Visualization of an Adenovirus Capsid-Incorporated HIV Antigen
نویسندگان
چکیده
منابع مشابه
CryoEM Visualization of an Adenovirus Capsid-Incorporated HIV Antigen
Adenoviral (Ad) vectors show promise as platforms for vaccine applications against infectious diseases including HIV. However, the requirements for eliciting protective neutralizing antibody and cellular immune responses against HIV remain a major challenge. In a novel approach to generate 2F5- and 4E10-like antibodies, we engineered an Ad vector with the HIV membrane proximal ectodomain region...
متن کاملEvidence for a new antigen within the adenovirus capsid.
Complement-fixation and radioactive-agglutination tests were performed on purified preparations of adenovirus type 5 using specific antisera against capsid antigens and an antiserum which can detect an antigen (the P antigen) in infected cells which has many of the properties of the T antigen associated with infection by the oncogenic adenoviruscs. A serological reaction was detected between pr...
متن کاملCritical Role of Autophagy in the Processing of Adenovirus Capsid-Incorporated Cancer-Specific Antigens.
Adenoviruses are highly immunogenic and are being examined as potential vectors for immunotherapy. Infection by oncolytic adenovirus is followed by massive autophagy in cancer cells. Here, we hypothesize that autophagy regulates the processing of adenoviral proteins for antigen presentation. To test this hypothesis, we first examined the presentation of viral antigens by infected cells using an...
متن کاملArchitecture of the Adenovirus Capsid.
Smith, Kendall O. (Baylor University College of Medicine, Houston, Tex.), Warren D. Gehle, and Melvin D. Trousdale. Architecture of the adenovirus capsid. J. Bacteriol. 90:254-261. 1965.-The capsids of adenovirus type 2 were fragmented by treatment with low concentrations of sodium lauryl sulfate. The clusters of capsomeres resulting from this treatment displayed characteristic patterns. Some o...
متن کاملAntigen capsid-display on human adenovirus 35 via pIX fusion is a potent vaccine platform
Durable protection against complex pathogens is likely to require immunity that comprises both humoral and cellular responses. While heterologous prime-boost regimens based on recombinant, replication-incompetent Adenoviral vectors (AdV) and adjuvanted protein have been able to induce high levels of concomitant humoral and cellular responses, complex manufacturing and handling in the field may ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2012
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0049607